Fig. 8: NLP-10 suppresses habituation to repeated blue light stimuli.
From: Hierarchical regulation of functionally antagonistic neuropeptides expressed in a single neuron pair
a–c Wild type (zxIs153 juIs164) and nlp-10(zx29) animals were exposed to blue light (470 nm, 1.3 mW/mm2) for 30 s four times, as indicated, while their locomotion was monitored by MWT. a Locomotion speed was plotted over time (seconds). b Speed during 1st (t = 301:330) to 4th (t = 1201:1230) illumination (v1 to v4) and (c) the difference between the v1 and v4 were plotted. Despite expressing Chrimson in AVK, experiments were conducted in the absence of ATR, representing the response derived from innate mechanisms within the animals. Data points overlap with Fig. 8d and 9a. In (b), *** indicates p < 0.001 (Two-tailed Welch test), ## and ### indicate p < 0.01 and p < 0.001 (Dunnett test against v1 of wild type) and $$$ indicates p < 0.001 (Dunnett test against v1 of nlp-10). In (c), * indicates p < 0.05 (Two-tailed Welch test), and ### indicates p < 0.001 (One-sample t-test). Approximately 50–200 animals were involved in each recording. d. Animals with indicated genotypes were analyzed as in a. Speed during v4 was plotted. Tukey test was performed. ***p < 0.001. Approximately 50–200 animals were involved in each recording. In timeseries plots, data are presented as mean values +/- SEM. In boxplots, the boxes extend from the Q1 to Q3, with the band inside the boxes representing the median. The whiskers extend to the smallest and largest values within 1.5 times the IQR. Source data are provided in ‘Source Data 1’ file.
