Fig. 6: Fibroblasts are an important source of chemokines in the tumor. | Nature Communications

Fig. 6: Fibroblasts are an important source of chemokines in the tumor.

From: Cancer-associated fibroblast subtypes modulate the tumor-immune microenvironment and are associated with skin cancer malignancy

Fig. 6

A Expression of immunomodulatory genes in iCAFs compared to healthy and neoplastic keratinocytes and melanocytes and interrogation for respective receptors in healthy and neoplastic keratinocytes and melanocytes as well as immune cells (n = 15). B Circular plots of selected receptor-ligand pairs from CellChat analysis, showing mCAF/iCAF as source cells (n = 15 donors). C Representative images of RNA ISH staining with probes against CXCL2, CXCL8, IL24, and COL1A1 of BCC, SCC and melanoma samples (n = 43 biologically independent tumor samples). D In vitro cytokine expression of NHDF after exposure to conditioned medium from NHDFs, VM08, VM15, VM26, VM19, VM25, and SCC13 cells for 72 h in comparison to the cytokine expression of the cancer cell lines VM08, VM15, VM26, VM19, VM25, and SCC13, and to primary melanoma-derived CAFs (pMel CAFs). Data from four independent experiments are presented as bar graphs showing mean values ± SD, overlayed with individual data points of the independent experiments. Statistical analysis by One-way-ANOVA and Tukey’s post hoc test for multiple comparison on log-transformed data. Significant comparisons to NHDFs are shown; Source data and exact p values are provided in the Source Data file. *p < 0.05, **p < 0.01, ***p < 0.001.

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