Fig. 7: Fibroblasts activate CD4+ and CD8+ T cells. | Nature Communications

Fig. 7: Fibroblasts activate CD4+ and CD8+ T cells.

From: Cancer-associated fibroblast subtypes modulate the tumor-immune microenvironment and are associated with skin cancer malignancy

Fig. 7

A Experimental setup of T cell assays shown in B and C. B Proliferation assessed by flow cytometry of CD4 or CD8 T cells upon co-culture with NHDFs pre-treated with conditioned medium from cancer cells, primary melanoma-derived CAFs (pMel CAFs) or cancer cells. C Upregulation of the early activation marker CD69 on CD4 or CD8 T cells after 24 h of co-culture with NHDFs pre-treated with conditioned medium from cancer cells, pMel CAFs or cancer cells. Data represented as fold change of percentages of cells positive for the indicated markers normalized to NHDFs. B, C n = 6 biologically independent samples for T cells only and untreated NHDFs, n = 5 biologically independent samples for pMel CAFs, and n = 3 biologically independent samples for T cells in co-culture with VM15, VM26, VM19, VM25. Data are presented as mean values ± SD. Statistical analysis in comparison to NHDFs or to T cells only by unpaired two-sided Student’s t test and Welch’s correction; B, C Source data and exact p values are provided in the Source Data file. *p < 0.05, **p < 0.01, ***p < 0.001. D Schematic summary of spatial distribution of distinct CAF subsets in human skin cancer.

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