Fig. 4: Universality of CAS-LNP. | Nature Communications

Fig. 4: Universality of CAS-LNP.

From: Charge-assisted stabilization of lipid nanoparticles enables inhaled mRNA delivery for mucosal vaccination

Fig. 4

a Representative aqueous AFM amplitude images and (b) force curves of SM102-LNP and CAS-LNP. c Young’s modulus and (d) the maximum force required to break LNP calculated from the force curves of individual LNP (n = 23 technical replicates). Data are shown as mean ± SD. Statistical significance was analyzed by unpaired two-tailed Student’s t test. e Zeta potentials of MC3-LNP, MC3-CAS-LNP, ALC0315-LNP, and ALC0315-CAS-LNP (n = 3 technical replicates). Data are shown as mean ± SD (f) Percentage of intact LNPs after nebulization (n = 3 technical replicates). Data are shown as mean ± SD. The statistical significance was analyzed by one-way ANOVA and Tukey’s multiple comparisons test. g Representative IVIS images of major organs and (h) quantitative analysis of lungs at 24 h post-administration (n = 4 biologically independent samples). Data are shown as mean ± SEM. The statistical significance was analyzed by one-way ANOVA and Tukey’s multiple comparisons test. i IVIS images of tracheas and lungs of dog and pig (j) at 3 h post-administration. Dog and pig were administered with 0.3 mg/kg of mFluc through the Aerogen Solo nebulizer with a custom nose cone. Figure 4i and j were created in BioRender. Lu, X. (2024) BioRender.com/s18b330. Source data are provided as a Source Data file.

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