Fig. 3: Horizontal transfer of a bacterial gene into Acrasis kona included post-transfer addition of a signal peptide sequence and introns, followed by gene duplication.

Two copies of a sequence-conserved protein of unknown function are found in A. kona and a scattering of other eukaryotes trace to Bacteria, indicating gene acquisition by horizontal gene transfer (HGT). A HGT from Bacteria to A. kona followed by gene duplication is indicated by maximum likelihood analysis of a 503 amino acid alignment. B Both A. kona sequences carry a 5’ signal peptide sequence and two identically-placed introns (shown for AKO1_005347), and (C) with one intron lying at the junction between a predicted signal peptide sequence and the mature protein coding sequence (highlighted in yellow).