Fig. 7: The Acrasis kona proteasome and its life-cycle dependent expression pattern compared to Dictyostelium discoideum AX4 (Ddi AX4).
Proteins were identified by BLASTp using human queries with hit strength indicated by color intensity (key at bottom right). Hits with low e-values (e−25 to e−5) were scored as present only if they showed > 50% query coverage (cvg). RNAseq length-corrected read numbers are shown for growing (RPKMGro) and aggregating (RPKMAgg) A. kona cells and similar time points for Ddi AX4 (RPKM0h and RPKM5h, respectively). Green and red arrows indicate substantially increased or decreased gene expression for aggregation overgrowth (DEAgg) and germination over aggregation (DEGerm) in A. kona and for 0h vs 1 h (DE0_1) and 2 h vs 5 h (DE2_5) hours of starvation in Ddi AX4. Accession numbers, BLASTp e-values, and DE values are in Supplementary Data 10. Taxon name abbreviations are as follows: A. kona (Ako), Aureococcus anophagefferens (Aan), Chlorella variabilis (Cva), D. discoideum AX4 (Ddi), Naegleria gruberi (Ngr), Saccharomyces cerevisiae S288c (Sce). DdiAX4 gene expression values were derived from Santhanam et al.11.
