Fig. 8: Developmental signaling pathways up-regulated in aggregating Acrasis kona. | Nature Communications

Fig. 8: Developmental signaling pathways up-regulated in aggregating Acrasis kona.

From: The Acrasis kona genome and developmental transcriptomes reveal deep origins of eukaryotic multicellular pathways

Fig. 8

A. kona accessions with substantially increased expression during aggregation (Aggup) and several key components with substantial (RPKM > 100) but not substantially-increased aggregation expression are shown in (A) graphical and (B) table format. The table is further split into external (B) and internal (Supplementary Table S7) signaling pathways. Predicted proteins are grouped into common signaling pathways (www.expasy.org) with corresponding colors in (A, B) and Supplementary Table S7. Signaling interactions are indicated as follows: canonical activation (solid arrows), predicted activation (dotted-line arrows), inhibition (red-blockhead arrows), and degradation (arrows capped with a red X). Second messenger molecules are shown in ovals as follows: cAMP (cyclic AMP), DAG (diacylglycerol), IP 3 (inositol 3-phosphate), Pi(4,5)P 2 (phosphoinositol 4,5-bisphosphate), AEA (anandamide), S1P (sphingosine 1-phosphate) and calcium (Ca + +). Protein names are abbreviated as follows: G-protein coupled receptor (GPCR), G-protein alpha (Ga), beta (Gb), and gamma (Gg) subunits, hybrid histidine kinase signal receptors (hybrid hisK), mitogen-activated protein kinase (MAPK), N-acyl-phosphatidylethanolamine (PLD), small GTPases (Arf, Rac, Rap, Ras and Rho), GTPase exchange factors (GEFs) and activators (GAPs), phosphatase (P*tase), tyrosine kinase (Yk’ase). Structure/function predictions for AKO1_000820 (GPCR) and AKO1_011986 (novel 1TM receptor) are shown in Supplementary Fig. S8. Tables in (B) are organized by general signaling pathways and show, from left to right, A. kona accession numbers, protein names, top BLASTp hit e-values, DE Log2 for aggregation overgrowth (Agg) and germination over aggregation (Germ), length corrected read numbers for aggregation (RPKM) and up-or down-regulation of Ddi AX4 homologs (green and red arrows, respectively). Details of sequence annotation, supporting evidence, and gene expression levels are shown in Supplementary Data 7 and 14.

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