Fig. 3: Disrupting the OMD accelerates the action potential firing of DRG neurons.

a Representative heatmap FRET images of small nociceptor DRG neurons before and after the acute β-cyclodextrin treatment. Summary data of the effects of β-cyclodextrin (n = 9 cells, **p = 3e-7) and WSC (n = 5 cells) on the FRET efficiency between CTxB 488/647 probes. Data shown are mean ± s.e.m., n = 7 for the control. One-way ANOVA (no adjustment) was used. b Summary data of the effect of β-CD (n = 5 cells, **p = 6e-5) and WSC (n = 8 cells) on the FRET efficiency between the L10-CFP and L10-YFP pair. Data shown are mean ± s.e.m., n = 9 cells for the control. One-way ANOVA (no adjustment) was used. c Representative spontaneous action potential firings of small DRG neurons before and after the acute β-CD treatment. d Summary data of the effects of β-CD (n = 17 cells, **p = 0.001) and WSC (n = 10 cells) on the number of the un-elicited action potential firings during the 1 s recording period, compared to the control (n = 15 cells). Data shown are mean ± s.e.m. One-way ANOVA (no adjustment) was used. e Summary data of current injection-elicited action potential firing frequency. Same data with 0 pA current injection and the same number of cells as in panel (d); **p = 0.009 with 20 pA current injection between the control and the β-CD conditions; **p = 0.004 with 40 pA current injection between the β-CD and WSC conditions; *p = 0.04 with 60 pA current injection between the β-CD and WSC conditions. Data shown are mean ± s.e.m., one-way ANOVA was used.