Fig. 4: Organization of the two microtubule asters by the microtubule/motor/tip tracker system.

a, b Schematics of the activity of minus-end directed motors and their combination with tip tracker in antiparallel microtubule overlaps. c In the model one motor/tip tracker unit is represented as EB scaffold in which EBs are connected via HSET to the antiparallel microtubule. d–f Pole-to-pole distances as a function of time for different ratios between motors alone and motor/tip tracker complexes. Gray lines are individual simulations, blue lines are averages. Microtubule growth was 2x faster than motor movement (Supplementary Fig. 2b). g Steady state pole-to-pole separation as a fraction of the motors/tip tracker complexes to all complexes in the system. Line is trendline. h Snapshot images of the simulations for the 90% motor/tip tracker complexes in the beginning and in the middle of the simulation (Corresponds to Supplementary Movie 6). Colors: Black – microtubules, Green – unbound HSET, Orange – unbound EB/HSET, Dark green – microtubule bound HSET, Red – microtubule bound EB/HSET. i–k Pole-to-pole distances as a function of time in the simulations for different ratios between HSET and EB/HSET in which EB/HSET was assumed to detach at forces exceeding 0.1 pN. Notations follow (d–f). Source data for this figure are provided as a Source Data file.