Fig. 1: Cryo-EM structure of human type I OSM receptor complex (hOSM/hgp130/hLIFR complex). | Nature Communications

Fig. 1: Cryo-EM structure of human type I OSM receptor complex (hOSM/hgp130/hLIFR complex).

From: Structures of complete extracellular assemblies of type I and type II Oncostatin M receptor complexes

Fig. 1

a Schematic representation of human OSM/gp130/LIFR complex assembly, with hLIFR, hgp130, and hOSM colored in purple, blue, and dark red, respectively. Transmembrane (TM) region and intracellular domains (ICD) of hLIFR and hgp130 were excluded from the constructs and were colored in gray. b Representative 2D class averages of human OSM/gp130/LIFR complex with full extracellular assembly. c 3.59 Å cryo-EM density map (Map 1) was overlaid with transparent Map 2 (3.99 Å). The assembly core regions of Map 1 (hOSM, hgp130 D2D3, and hLIFR D3D4) were colored as in (a) while the remaining parts of the map were colored in gray. d The atomic model of human OSM/gp130/LIFR complex assembly core region (hOSM, hgp130 D2D3, and hLIFR D3D4) manually built and refined against Map 1 was shown in cartoon form in two orientations. Glycosylation sites in this region were highlighted in stick representation. e 3.99 Å cryo-EM density map (Map 2) of human OSM/gp130/LIFR complex colored as in (a). f Model of complete extracellular assembly of human OSM/gp130/LIFR complex depicted in C-alpha ribbon traces. g Side view of the full extracellular assembly model in transparent Map 2. Approximate distances between the bottom centers of hLIFR and hgp130 juxtamembrane domains is estimated.

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