Fig. 4: Cryo-EM structure of mouse type II OSM receptor complex (mOSM/mgp130/mOSMR complex). | Nature Communications

Fig. 4: Cryo-EM structure of mouse type II OSM receptor complex (mOSM/mgp130/mOSMR complex).

From: Structures of complete extracellular assemblies of type I and type II Oncostatin M receptor complexes

Fig. 4

a Schematic representation of mouse OSM/gp130/OSMR complex assembly, with mOSMR, mgp130, and mOSM colored in gold, light blue, and brown, respectively. Transmembrane (TM) region and intracellular domains (ICD) of mOSMR and mgp130 were excluded from the constructs and were colored in gray. b Representative 2D class averages of mouse OSM/gp130/OSMR complex with full extracellular assembly. c 3.46 Å cryo-EM density map (Map 1) was overlaid with transparent Map 2 (3.67 Å). The assembly core regions of Map 1 (mOSM, mgp130 D2D3, and mOSMR D2D3) were colored as in (a) while the remaining parts of the map were colored in gray. d The atomic model of mouse OSM/gp130/OSMR complex assembly core region (mOSM, mgp130 D2D3, and mOSMR D2D3) manually built and refined against Map 1 was shown in cartoon form in two orientations. Glycosylation sites in this region were highlighted in stick representation. e 3.67 Å cryo-EM density map (Map 2) of mouse OSM/gp130/OSMR complex colored as in (a). f Model of complete extracellular assembly of mouse OSM/gp130/OSMR complex depicted in C-alpha ribbon traces. g Side view of the full extracellular assembly model in transparent Map 2. Approximate distances between the bottom centers of mOSMR and mgp130 juxtamembrane domains is estimated.

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