Fig. 1: Precursor-independent MS/MS-based N-glycome profiling.
From: Non-targeted N-glycome profiling reveals multiple layers of organ-specific diversity in mice
A top panel: Biosynthetic pathway leading to complex-type and hybrid-type N-glycans. lower panel: Precursor mass over chromatographic retention time plots MS/MS spectra. Each data point represents a single N-glycan derived MS/MS spectrum (i.e containing diagnostic fragment ions at 224.1 amu and one of the following signals: 274.1 amu for Neu5Ac-sialylation, 290.1 amu for Neu5Gc-sialylation, 512.2 amu for fucosylation, and 792.3 amu for bisecting GlcNAc). B Relative spectral count. Data were normalized to the total number of N-glycan-derived MS/MS spectra (i.e. containing 224.1 amu) in the respective tissues. All tissues were analyzed in duplicates. Numeric results of the spectral counting are provided in Supplementary Table 1. WAT - white adipose tissue.
