Fig. 2: Comparative N-glycome analysis.
From: Non-targeted N-glycome profiling reveals multiple layers of organ-specific diversity in mice
A Semi-quantitative histograms of SNOG-filtered LC-MS data of selected tissues. LC-MS data were charge-deconvoluted, deisotoped, and summed across the chromatographic time-range using custom code. Precursor mass-to-intensity arrays are shown in the range of 1000−4000 Da (bin-width = ±0.05 amu). Representative, tissue-specific N-glycan compositions are indicated by their precursor mass (i.e. [M + H]+ in amu) and tentative structure assignments using the Symbol Nomenclature for Glycans (SNFG). Signals below a cumulative intensity threshold of 5E + 6 and a SNOG-score of 0.03 were removed. Inset pie charts show the N-glycan compositional fractions of the respective N-glycome (green: oligomannosidic- (OM), purple: sialylated (including both Neu5Ac and Neu5Gc-sialylated glycans), yellow: undecorated/neutral N-glycans). B N-glycan compositions of tissues not included in Panel A. WAT- white adipose tissue, Lymph N - lymph nodes, Mammary G. - mammary gland.
