Fig. 3: MHC-I immunopeptidome exhibits distinct cysteine reactivities.
From: A platform for mapping reactive cysteines within the immunopeptidome
a Schematic representation of the immunopeptidomics workflow. b The number of 8-13-mer MHC-I-associated peptides in MT2 and BV173 cells treated with 50 µM of MS-DTB. The result is a representative of two experiments (n = 2 independent replicates). c Motif analysis of all 9-mer MHC-I-bound antigens, cysteine-containing 9-mer MHC-I-bound antigens, cysteinylated 9-mer MHC-I-bound antigens, and MS-DTB-modified 9-mer MHC-I-bound antigens. d Distribution of all cysteines, cysteinylated cysteines, and MS-DTB-modified cysteines on 9-mer peptide antigens in MT2 and BV173 cells. e Venn diagram of MHC-I-bound antigens with unmodified, cysteinylated, and MS-DTB-modified cysteines. Source data are provided as a Source Data file.
