Fig. 3: Cellular landscape of the peripheral nervous system.

A Dotplot of mean log-normalised expression values of marker genes amongst cells of the fetal peripheral nervous system. Point size shows the percentage of cells with non-zero expression. Coloured bars correspond to cell types represented in B. SC, Schwann cell; SN, sensory neuron; Pct. Exp, percentage of expressing cells; Av. Exp, average expression in cell type. B Spatial heatmaps showing cellular abundancy scores (left panel) in Post-conception week 9 peripheral nervous system calculated using cell2location and marker gene expression for those cell types (right panel). Boxes on the left panel denote regions represented in two panels on the right. Symp, sympathetic neurons. C Spatial heatmaps showing cellular abundancy scores (left panel) in PCW9 peripheral nervous system calculated using cell2location and marker gene expression for those cell types (right panel). Box on the left panel denote regions represented in the panel on the right. SC, Schwann cell. D Uniform manifold approximation and projection (UMAP) plot visualising single-cell RNA sequencing data cell types (left panel) and contribution of each post-conception week stage to the manifold (right panel). PCW, post conception week;Teno-P, tenocyte progenitor; IVD, intervertebral disc; HCC, hypertrophic chondrocyte; PHC, prehypertrophic chondrocyte; DRG, dorsal root ganglia; CC, chondrocyte; CNS, central nervous system. Colours in the bar chart represent cell types in the adjacent colour key. E Stacked bar charts showing the fraction of each cell type in the mesenchymal (left panel), neural crest (centre panel) and neuroglial (right panel) compartments at each sample stage. CC, chondrocyte; SC, schwann cell; SN, sensory neuron; PCW; post-conception week. Source data are provided as a Source Data file.