Fig. 3: Genomic structure of centromeric regions.
From: Telomere-to-telomere genome assembly of a male goat reveals variants associated with cashmere traits
a Schematic representation showing the sequence compositions in the centromeric region of chromosome 1 (Chr01). From top to bottom: RNA-seq, expression (read counts in 1-kb windows) of genes in the pericentromeric region as indicated by an arrow on the top; Methylation by HiFi and ONT in 20-kb windows; ChIP-seq for CENP-A protein enrichment based on Phospho-CENP-A (Ser7) antibodies in 5-kb windows; Centromeric satellites of SatI (blue), SatII (orange) and SatIII (green); the occurrence of LINE, SINE, and LTR; and Entropy that was calculated for sequence complexity with low values for centromeric regions. The pairwise 10-kb sequence identity (%) heatmap in centromeric region is shown below, with color key in the bottom left corner. The boundary between centromeric and pericentromeric regions is close to the coordinate 9 Mb on Chr01. b Length distribution of centromeric regions (top) and proportions of three centromeric satellite units (bottom) on all the autosomes and the X chromosome. c, FISH results for probes of CenY (white), SatII (green) and SatIII (red). CenY is shown uniquely on the Y chromosome, with experimental replicates (n = 5). The enlarged image of CenY probe (white) and its unique binding to the Y chromosome is shown in a small box in white line in the top left panel.
