Fig. 10: Application of ReaL-MGE 1.0 to improve intracellular malonyl-CoA titer of P. putida. | Nature Communications

Fig. 10: Application of ReaL-MGE 1.0 to improve intracellular malonyl-CoA titer of P. putida.

From: ReaL-MGE is a tool for enhanced multiplex genome engineering and application to malonyl-CoA anabolism

Fig. 10

a FACS sorting for GFP expression from the FapR biosensor in P. putida wild type and recombinants. b Fluorescence intensity was determined for 192 candidates leading to the choice of clone 177# (P. putida.C177) for further work in P. putida. c Evaluation of malonyl-CoA in P. putida wild type and P. putida.C177. d Comparison of genome sequence of P. putida.C177 and P. putida wild type. Green arrows, malonyl-CoA metabolic pathway promoter replacements; purple arrows, LVA insertion. Values are means of the biological replicates, and the error bars indicate the standard deviations of all (n = 5) biological replicates. P values were obtained using the two-tailed Student’s t test: ****P < 0.0001.

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