Fig. 2: Manipulation of ExoVII improves homologous recombination in different bacteria.
a Host’s xseB gene was cloned into the recombinases to create pBBR1-PRha-recombinases-xseB-Km. b A conventional recombineering assay (insertion cm resistance gene into the genome using 100 bp homology arm) was used to evaluate the impact of the loss of xseA (ΔxseA), the overexpression of xseB (P-xseB) and combination of ΔxseA and P-xseB in E. coli BL21. c Manipulation of ExoVII in S. brevitalea DSM7029 (insertion of genta resistance gene into the genome using 100 bp homology arm). d Manipulation of ExoVII in P. putida KT2440 (insertion of genta resistance gene into the genome using 100 bp homology arm). Values are means of the biological replicates, and the error bars indicate the standard deviations of all (n = 3) biological replicates. P-values were obtained using the two-tailed Student’s t test: *P < 0.1, **P < 0.01, ***P < 0.001, ****P < 0.0001.
