Fig. 4: Development and characterization of CTR/CTA 2.0. | Nature Communications

Fig. 4: Development and characterization of CTR/CTA 2.0.

From: Repurposing endogenous type I-E CRISPR-Cas systems for natural product discovery in Streptomyces

Fig. 4: Development and characterization of CTR/CTA 2.0.The alternative text for this image may have been generated using AI.

a Schematics of CRISPR-based transcriptional repressor/activator 2.0 (CTR/CTA 2.0) in this work. The Cascade consists of 5 genes (casA-casB-cas7-cas5-cas6). The scRNA plasmid consists of a scRNA cassette with crRNA coupled to the MS2 sequence, and an activator domain under the control of the kasO*p promoter. b Repression results for targeting the act-orf4 coding sequence using the CTR 2.0. c Repression results for targeting the redQ coding sequence using the CTR 2.0. d Activation result for targeting the act-orf4 promoter region using the CTA 2.0. Control (For 4b and 4c, control represents the wild-type strain of S. coelicolor; For 4 d, control represents the wild-type strain of S. lividans).

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