Fig. 7: Activation of C11 biosynthetic gene cluster in Nh. DSM 44494. | Nature Communications

Fig. 7: Activation of C11 biosynthetic gene cluster in Nh. DSM 44494.

From: Repurposing endogenous type I-E CRISPR-Cas systems for natural product discovery in Streptomyces

Fig. 7: Activation of C11 biosynthetic gene cluster in Nh. DSM 44494.The alternative text for this image may have been generated using AI.

a CrRNA targeting the promoter region of the C11-ctg1-16_96 gene are shown. b HPLC analysis for activating C11 BGC using the CTA 2.0. c The structure of the compound C11-Legonmycin C-E. A2-4 (Activation strains with kasO*p driven the expression of the Sav Cascade and MS2-coupled SoxS domain); Control (The wild type strain of Nh. DSM 44494). Source data are provided as a Source Data file.

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