Fig. 1: Role of AmpG in peptidoglycan recycling and antibiotic resistance. | Nature Communications

Fig. 1: Role of AmpG in peptidoglycan recycling and antibiotic resistance.

From: Cryo-EM characterization of the anydromuropeptide permease AmpG central to bacterial fitness and β-lactam antibiotic resistance

Fig. 1: Role of AmpG in peptidoglycan recycling and antibiotic resistance.

a Under physiological conditions, mature PG is processed into GlcNAc-1,6-anhydroMurNAc peptides (primarily tetrapeptides) that are transported across the bacterial inner membrane to the cytosol by AmpG for recycling. An intermediate in this normal PG biosynthetic pathway, UDP-MurNAc-pentapeptide, binds to AmpR, which represses production of the class C β-lactamase AmpC. However, in the presence of β-lactams, inhibition of PBPs leads to an accumulation of hydrolyzed PG pentapeptide fragments, which are transported to the cytosol by AmpG, converted to 1,6-anhydroMurNAc-pentapeptides by NagZ, bind AmpR and induce expression of ampC. Secreted AmpC hydrolyzes β-lactams, providing antibiotic resistance10. b Chemically synthesized GlcNAc-1,6-anhydroMurNAc derivatives made for this study.

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