Fig. 8: Cav1 Y14 phosphoacceptor modulates adipose tissue mechanical properties and adipocyte mechanical response to lipid droplet expansion.
From: Plasma membrane remodeling determines adipocyte expansion and mechanical adaptability
a Median Young’s Modulus for visceral adipose tissue explants. p values: 0.036 (HFD, 6 animals), 0.801 (ND, 11 animals). b Median Young’s Modulus for adipocytes differentiated in vitro. p value: 0.01, 22+/+, 20 Y14F/Y14F cells from 4 animals per genotype. c Representative examples of Flipper-TR FLT in adipocytes after 120 h of HF treatment and their manually segmented membranes. Scale bar: 20 μm. d FLT of adipocyte membrane staining of Flipper-TR as a function of adipocyte area. 24+/+, 32 Y14F/Y14F cells for iso-osmotic, 17+/+, 18 Y14F/Y14F for hyper-osmotic. Average FLT and area for different z sections ±SEM represented. e LDH activity in culture media after compression. Lines connect contralateral depots of the same animal, p values: 4.2 × 10−5, 0.028. f LDH release increment calculated as in Fig. 4c, p value = 1. Sample size for e, f 10 animals per genotype. g Fragility assay performed with DMEM (iso) or 1/10 DMEM dilution (hypo) on in vitro differentiated adipocytes after incubation with 250 nM oleate (HF) or vehicle (LF). Sample size: 24 independent +/+ cell plates for HF and 20 for LF; 18 independent cell plates for Y14F/Y14F HF and LF iso and 17 for LF hypo. Effect of diet on mean adipocyte circularity (h) and area (i) in 45-week old animals. p values for the effect of diet in WT circularity and area are 0.028 and 6.50 × 10−14, respectively; p values for diet-genotype interaction are 0.904 for circularity and 0.132 for area. The sample size for h and i: For ND, 30 animals +/+ and 29 Y14F/Y14F; for HFD, 15 animals per genotype. Cav1+/+ plots and images for a–f, h, i where already presented in Fig. 4, and are repeated here for clarity. For a, b, f p values are for pairwise t-test with Bonferroni corrections, and paired t-test for e, all two-sided. For g–i p-values correspond to t-test for regression coefficients (two-sided). p-value codes: * <0.05, **<0.01, ***<0.001. All boxplots show first, second and third quantiles, and whiskers extend from Q1-1.5×IQR to Q3+1.5×IQR. Source data provided as a SourceData.zip.
