Fig. 5: Deletion of either TNFR1 or TNFR2 reduces NK cell terminal maturation. | Nature Communications

Fig. 5: Deletion of either TNFR1 or TNFR2 reduces NK cell terminal maturation.

From: Dichotomous outcomes of TNFR1 and TNFR2 signaling in NK cell-mediated immune responses during inflammation

Fig. 5: Deletion of either TNFR1 or TNFR2 reduces NK cell terminal maturation.

Transgenic Ncr1cre, Ncr1creTnfr1fl/fl, and Ncr1creTnfr2fl/fl mice were infected with S. Typhimurium, and NK cells analyzed by scRNA-seq on day four post-infection. a UMAP plot of NK cells colored by Seurat clusters, where each dot represents a single cell. b Dot plot showing the relative abundance of signature genes from each cluster. c UMAP plot of scRNA-seq differential abundance between WT and Ncr1creTnfr1fl/fl by Milo, where sampled neighborhoods are colored according to logFC. d Beeswarm plot showing the distribution of logFC between WT and Ncr1creTnfr1fl/fl in neighborhoods separated by Seurat clusters. e UMAP plot of scRNA-seq differential abundance between WT and Ncr1creTnfr2fl/fl by Milo, where sampled neighborhoods are colored according to logFC. f Beeswarm plot showing the distribution of logFC between WT and Ncr1creTnfr2fl/fl in neighborhoods separated by Seurat clusters. For c–f, colored neighborhoods represent statistically significant differences (FDR < 0.05 by quasi-likelihood F-test). Data from a single experiment (n = 4 Ncr1cre, 4 Ncr1creTnfr1fl/fl, and 3 Ncr1creTnfr2fl/fl). Abbreviations: WT, wild-type; Nhood, neighborhood; logFC, log fold change.

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