Fig. 6: Depletion of rsmA and rsmG, but not other methyltransferases, leads to increased resistance to kasugamycin and streptomycin. | Nature Communications

Fig. 6: Depletion of rsmA and rsmG, but not other methyltransferases, leads to increased resistance to kasugamycin and streptomycin.

From: Native RNA nanopore sequencing reveals antibiotic-induced loss of rRNA modifications in the A- and P-sites

Fig. 6: Depletion of rsmA and rsmG, but not other methyltransferases, leads to increased resistance to kasugamycin and streptomycin.

A Median growth curves from four biological replicates of different E. coli strains (wild type, rsmA knockout, rsmG knockout and rsmE knockout) across a range of antibiotic (streptomycin, upper panels; kasugamycin, lower panels) concentrations. Data for each biological replicate was computed as the median of three technical replicates. Growth curve data was fitted to a logistic curve. See also Figure S27 for growth curves in additional E. coli strains tested (rsmB, rsmD, rsmH and rsmJ knockout strains). B Barplots depicting Median Area Under the Curve (AUC) difference in growth curves between wild type and knockout strains (KO-WT), for every KO strain and across all antibiotic concentrations tested. In bold, the strains that are reported to have an increased resistance to each specific antibiotic. Error bars indicate  standard deviation of four biological replicates. Source data are provided as a Source Data file.

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