Fig. 6: ALK neurons functioned in modulating female post-mating yeast preference.
From: Mating reconciles fitness and fecundity by switching diet preference in flies
a The intersection between CrzR neurons and LK neurons showed the expression pattern of ALK neurons in the female central nervous system. The white arrow indicated the cell bodies of ALK neurons. Green: GFP. Magenta: nc82. The scale bar represented 50 μm. b Yeast preference of females when ALK neurons were silenced with Kir2.1. Mean ± SEM. *p < 0.05, n.s. not significant, two-tailed unpaired t-tests. c Ca2+ signal of female ALK neurons in different mating states. The right panel showed the relative fluorescence intensity (FGcamp6s/FtdTom) of female ALK neurons in different mating states. Mean ± SEM. **p < 0.01, n.s. not significant, two-tailed unpaired t-tests. n = 15,17. d Yeast preference of females when knocking down different neuropeptides in female LK neurons. Mean ± SEM. *p < 0.05, n.s. not significant, two-tailed unpaired t-tests. e The voltage signal of female FB-LAL neurons in different mating statuses. The left panels represent the signals of Arclight, and the right panels represent the signals of tdTomato. White dotted lines outline the brain. The calibration bar ranges from 0–255. Scale bar, 10 μm. f The relative fluorescence intensity (FArclight/FtdTom) of female FB-LAL neurons in different mating statuses. Mean ± SEM. n.s. not significant, two-tailed unpaired t-tests. n = 13, 15. g Graphic model of a sex-specific mechanism where different peptides and their downstream signaling pathways play crucial roles in regulating the post-mating modulation of yeast preference. Source data are provided as a Source Data file.
