Fig. 7: PDCD6 interacts with LDHA.
From: PDCD6 regulates lactate metabolism to modulate LC3-associated phagocytosis and antibacterial defense
a, b Immunoblotting of p-LDHA in wild-type and PDCD6-knockdown (KD) THP-1 cells treated with L. monocytogenes (a) or S. Typhimurium (b) for the indicated periods. Numbers indicate the densitometric values of p-LDHA signals relative to actin. c, d LDH activity in Pdcd6fl/fl and Pdcd6Δmye bone marrow-derived macrophages (BMDMs) stimulated with L. monocytogenes (c) or S. Typhimurium (d) for the indicated times. e PDCD6 binding partners with high scores were identified through mass spectrometry. f, g Total PDCD6 was immunoprecipitated from BMDMs challenged or not with L. monocytogenes (f) or zymosan (g) for 2 h, followed by immunoblotting with anti-LDHA antibody. The averages of n = 3 (c, d) biologically independent samples are shown. Data are shown as the mean ± SEM. Statistical significance in (c, d) was determined using the t test (and nonparametric tests). The data presented in (a–d, f, g) are representative of three independent experiments.
