Fig. 2: Multi-omic analyses of Nr1h2 activation in conferring expanded pluripotency.

a Representative images of NrESC-derived blastoids harvested at Day 5 of differentiation. Scale bar, 150 µm 25 independent experiments were repeated with similar results. b Quantification of blastoid formation efficiency from different passages of NrESC. X-axis denotes number of passages cultured in presence of T09, where +0 referred to starting ESC. c Brightfield images of ESC and NrESC. Scale bar, 100 µm. 100 independent cultures were repeated with similar results. d A schematic diagram showing the multi-omic approach of the molecular characterization of NrESC. Created in BioRender.com e Principle-component analysis (PCA) of bulk RNA-seq data from ESC, NrESC (Passage 16), and EPSC along with single-cell RNA-seq data from development stage based average expression values. f Average Nr1h2 ChIP-seq signal comparison between NrESC (Passage 16) and ESC on Nr1h2 binding peaks with H3K27ac increases in NrESC (Passage 16). g Average H3K27ac ChIP-seq signal comparison between NrESC and ESC on Nr1h2 binding peaks with H3K27ac increases in NrESC. h Heatmap showing the representative genes upregulated in NrESC with differential H3K27ac peaks. i Representative genes upregulated in NrESC with increased H3K27ac signal in comparison to ESC. Source data are provided as a Source Data file.