Fig. 7: RNF10 promotes 40S dissociation upon RPL KD. | Nature Communications

Fig. 7: RNF10 promotes 40S dissociation upon RPL KD.

From: E3 ubiquitin ligase RNF10 promotes dissociation of stalled ribosomes and responds to ribosomal subunit imbalance

Fig. 7

a Polysome profile analysis of HeLa, HeLa-KO+RNF10-WT and HeLa-KO+RNF10-Mut (C225S/C228S) cells 16 h after KD of RPL7 (siRNA S301) or RPL11 (S304) in comparison to control KD (S75); the yellow area is enlarged on the right side. b Ribosomal half-mers (2.5-mer/disome ratio) were quantified from polysome profiles as in panel (a), shown are mean values ± SD (n = 3 biological replicates; p-values determined by two-tailed, paired t-test). c Polysome profile analysis in parental HeLa cells and HeLa-KO+RNF10-WT cells following KD of RPL7 (S301) for different periods of time; profiles are shown separately in the yellow area on the right side. Source data are provided as a Source Data file.

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