Fig. 5: Functional profiles of root-associated microbiomes and metabolome profiling in roots. | Nature Communications

Fig. 5: Functional profiles of root-associated microbiomes and metabolome profiling in roots.

From: Root hair developmental regulators orchestrate drought triggered microbiome changes and the interaction with beneficial Rhizobiaceae

Fig. 5

a Principal coordinates analysis (PCoA) of the KEGG Orthology (KO) functions based on Bray–Curtis dissimilarity [calculated using TPM (transcripts per kilobase million) counts of KOs (PERMANOVA performed by Adonis, n = 3)]. b, c Volcano plots visualizing the functional genes enriched or depleted (adjusted P < 0.05) in (b) rsl2 rsl4, or (c) gl2 compared to Col-0. Red and blue dots represent the enriched genes in root hair mutants and Col-0, respectively. Labels of each gene were annotated based on the KEGG database. Differential analysis with a two-sided test was used for the statistical analysis. d Linear discriminant analysis (LEfSe) identified biomarker function pathways within each genotype. The KEGG pathways with significant differences (LDA score > 1 and P < 0.05) among genotypes were listed. e The flow plot showed the relative abundance of metabolite classes in the root hair mutants and Col-0. Stars represent the specific metabolites in root hair mutants that significantly differ from Col-0 (two-sided Student’s t test). *, **, ***: represent P < 0.05, P < 0.01, P < 0.001, respectively. f, g Heatmap visualizing the differences in the relative abundance of individual metabolite related to (f) proline and arginine derivatives and (g) flavonoids. The heatmap is colored by normalized Z scores.

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