Fig. 3: mIHC profiling and LMD capture of single cells in the pancreatic TME. | Nature Communications

Fig. 3: mIHC profiling and LMD capture of single cells in the pancreatic TME.

From: Multimodal single cell-resolved spatial proteomics reveal pancreatic tumor heterogeneity

Fig. 3: mIHC profiling and LMD capture of single cells in the pancreatic TME.The alternative text for this image may have been generated using AI.

a Spatial proteomics workflow of the SCPro. b Multiplexed immunohistochemical whole-slide image of a 4-μm-thick KPf/fC mouse tissue section. The color dot showing the representative LMD cutting position of distinct cell types (n = 3 biological replicates). Scale bar, 1 mm. c Representative tissue cytometry charts of the PDAC-1 region. d Workflow of the ROI selection, cell typing and cell mask generation, and image alignment based on the real-time image of LMD. Scale bar, 300 μm, 20 μm, 300 μm, 100 μm, and 20 μm for the images from left to right, respectively. e Automated LMD with single-cell resolution. The images shown are representative of 3 independent experiments. Scale bar, 10 μm. EpCAM (epithelial cells); CD45 (immune cells); αSMA (fibroblasts); DAPI (nuclei). PanIN pancreatic intraepithelial neoplasm, PDAC pancreatic ductal adenocarcinoma, ROI region of interest.

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