Fig. 1: The culture of gastruloids for 11 days allows the transcription of markers of the CPM and their derivatives.
From: Gastruloids are competent to specify both cardiac and skeletal muscle lineages
a Experimental scheme of the generation of gastruloids from mouse embryonic stem cells (mESCs). Gastruloids were cultured in N2B27 up to 11 days, with the addition of small molecules from day 4 to day 7 as indicated below the time line. b Representative images of gastruloids at day 3, day 4, day 5, day 6, day 7 and day 11. Arrowheads indicate beating areas. c Percentage of beating gastruloids per experiments (n = 8 independent experiments). Data are presented as mean percentage ± SEM. d Scheme of CPM specification in mouse toward the myogenic fates. Created in BioRender. Lescroart, F. (2024) https://BioRender.com/m68f097. Markers of each state are indicated in italics. e–g Expression profiles of Mesp1 (n = 9), Isl1 (n = 3) and Tbx1 (n = 6) (e), Tnnt2 (n = 9), Myh7 (n = 3) and Myl7 (n = 6) (f) and Tcf21 (n = 8), Myod (n = 7) and Myf5 (n = 5 independent experiments) (g) throughout the culture of gastruloids as measured by quantitative RT-PCR. Results are normalized to the expression of Tbp. Fold changes are represented relative to expression on day 0. Data are presented as mean values ± SEM.
