Fig. 6: Myogenic trajectories are found in gastruloids over time.
From: Gastruloids are competent to specify both cardiac and skeletal muscle lineages
a UMAP representation of all gastruloid cells merged from day 4 (red), day 5 (green), day 6 (blue) and day 11 (purple). Numbers represent identified clusters of day 11 (as defined in Fig. 3g). b, c Fate probabilities towards the cardiomyocyte macrostate (b) or towards the skeletal myoblast macrostate (c). Scale bars represent absorption probabilities. Numbers represent key cardiomyocytes and myoblast clusters of day 11. d URD trajectories showing transition from cells at day 4 (root) towards cells at day 11 (tips). Framed letters represent branches along the tree. The dotted box represents the lineage branch detailed in (f–o). e–i Expression of Isl1 (e), Tcf21 (f), Myl7 (g), Myl2 (h) and Myog (i) across URD trajectory. Scale bars represent expression levels. j–n Representation of clusters’ cells across URD trajectory. Red dots represent cells of the cardiopharyngeal mesoderm (CardioPharyng. mes.) cluster 1 of day 5 (in j, Fig. 3c, cluster 1), of the cardiopharyngeal mesoderm (CardioPharyng. mes.) cluster 2 of day 6 (in k, Fig. 3e, cluster 5), of the cardiopharyngeal mesoderm (CardioPharyng. mes.) cluster 3 of day 6 (in l, Fig. 3e, cluster 8), of the myoblast cluster 1 of day 11 (in m, Fig. 3g, cluster 16) and of the myoblast cluster 2 of day 11 (in n, Fig. 3g, cluster 18).
