Fig. 2: Seed formation and pollen tube germination is partially abolished in Arabidopsis atg5 and atg7 mutants.

a Representative images of siliques harvested from one branch of inflorescence of Arabidopsis WT, atg5-1, atg7-2, atg7-3, atg5-1/atg7-2, pATG5::GFP-ATG5/atg5-1 and pATG7::GFP-ATG7/atg7-2. Scale bars = 2 mm. b Statistical calculation of seeds setting rate in WT, atg5-1, atg7-2, atg7-3 atg5-1/atg7-2, pATG5::GFP-ATG5/atg5-1 and pATG7::GFP-ATG7/atg7-2. Statistical analysis was performed using two-tailed paired Student’s t-test, with n = 50 independent experimental replicates (error bars ± SD, ∗∗∗p < 0.001, ns, no significant). Source data are provided in Source data file. c Representative time-lapse images of in vivo pollen tube germination and growth of WT, atg5-1, atg7-2 and atg5-1/atg7-2 after pollination for 2 and 10 h respectively. Dashed lines indicate the farthest position that pollen tubes could reach into the styles. Scale bars = 400 μm. d Statistical calculation of the maximum pollen tube length in the styles of WT, atg5-1, atg7-2 and atg5-1/atg7-2 after pollination for 2, 4, 6 and 10 h were performed using two-tailed paired Student’s t-test respectively, with n = 50 independent experimental replicates from each sample (error bars ± SD, ∗∗∗p < 0.001 ns, no significant). Source data are provided in Source data file. e Representative time-lapse images of in vitro Arabidopsis pollen tube germination and growth of WT, atg5-1, atg7-2 and atg5-1/atg7-2 for 2, 4 and 6 h respectively. Scale bars = 200 μm. f, g Statistical analysis of the in vitro pollen germination ratio (f) and tube length (g) of WT, atg5−1, atg7-2 and atg5−1/atg7-2 were performed using two-tailed paired Student’s t-test For the pollen germination ratio, with n = 19 independent replicates were analyzed, while for the tube length, n = 200 independent replicates were analyzed for each sample (error bars ± SD, ∗∗∗p < 0.001, ns, no significant). Source data are provided in Source data file.