Fig. 3: Schwann cell C5aR1 mediates endometriosis-associated mechanical allodynia and neuroinflammation.
From: Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis
Representative images of SOX10 and C5aR1 (#orb393229, rabbit polyclonal, Biorbyt Ltd, 1:100) co-expression in sciatic (a) and trigeminal (b) nerve tissue from C57BL/6 J female (B6) mice, (n = 4 independent experiments) (Scale bar, 50 μm). (c) C5aR1 mRNA relative expression in primary mouse and human Schwann cells (n = 3 independent experiments). d Schematic representation of AAV-(loxP-shRNA)-C5aR1 vector pre- and post-Cre switch. e Representative images and cumulative data (Rcoloc) of SOX10 and C5aR1 (#orb393229, rabbit polyclonal, Biorbyt Ltd, 1:100) co-expression in sciatic and trigeminal nerve tissue in Plp-AAV-C5aR1 and Control mice (n = 4 independent experiments) (Scale bar, 50 μm). f Time-dependent periorbital (PMA), hind paw (HMA), and abdominal (AMA) mechanical allodynia in endometriotic (endo) or Sham Plp-AAV-C5aR1 and Control mice. (n = 8 mice per group). g Representative images and cumulative data of F4/80+ cells in sciatic and trigeminal nerve tissue in endo or Sham Plp-AAV-C5aR1 and Control mice. (n = 4 independent experiments) (Scale bar, 50 μm, dashed lines, perineurium). h Representative images and cumulative data of transmigrated macrophages after stimulation of human and mouse Schwann cells with C5a or vehicle (Veh) and in the presence of DF2593A (DF25) or Veh (n = 6 independent experiments). Data are mean ± s.e.m. c, Student’s t-test, f, 2-way, g, h 1-way ANOVA, Bonferroni correction; ****P < 0.0001 vs. Sham/Control, ####P < 0.0001 vs. Endo/Control. Source data are provided as a Source Data file.
