Fig. 6: Modulation of the dsDNA unwinding by Cas12e orthologs at low and high NaCl concentrations.
From: Cas12e orthologs evolve variable structural elements to facilitate dsDNA cleavage
a Cas12e effector medicated R-loop formation of the dsDNA duplex at low salt concentration. Orange dot, positively charged ion; blue dot, negatively charged ion. Green module, REC lobe; pink module, NUC lobe. b dsDNA unwinding and complete R-loop formation performed by NTSB-containing Cas12e and NTSB-lacking Cas12e at high salt concentration. Purple module, NTSB domain or loops. The thickness of the blue arrowed lines represents the likelihood of reaction occurrence. c The diversities of Cas12e and its cognate sgRNA. Different elements colored dark purple were attained based on the bilobed configuration. The sgRNA scaffolds (in black) with varying details in base pairing (colored green) indicate various sgRNAs. The PAM region on the dsDNA target is colored in yellow. Red triangles indicate the cleavage.
