Fig. 3: REV and STM expression patterns in LCR4m transgenic lines.
From: miR394 and LCR cooperate with TPL to regulate AM initiation
Continuous transverse sections and longitudinal sections showing pSTM::STM-Venus (green) expression and chlorophyll autofluorescence (red) in the shoot apex of the WT (a) and p35S::LCR4m (b) plants. STM was activated in AMs of WT plants (white arrows) but maintained low expression levels in the leaf axils of p35S::LCR4m plants (yellow arrows). Continuous transverse sections and longitudinal sections of pREV::REV-Venus (c) and pREV::REV-Venus p35S::LCR4m (d) shoot apex. REV (green) signals were strong in the AMs (white arrow) of the WT but were extremely weak in the leaf axils of p35S::LCR4m (yellow arrows). The asterisks represent the SAM, the arrows represent AMs, and the dashed lines represent the leaf axils where AMs were initiated. The numbers in the upper right corner represent the relative distances from the shoot apex. All the plants were grown under short-day conditions for 30 days. Scale bars, 100 μm. At least three samples were analyzed in (a–d) with similar results for each experiment.
