Fig. 1: Structural determination of the HsmortalinR126W-GrpEL1 complex.
A Hsp70 proteins collaborate with co-chaperones to perform substrate capture and release. Mitochondrial Hsp70 (mortalin) interacts with a J-protein partner to capture substrates and hydrolyze ATP. Once substrate-bound, mortalin interacts with GrpE-like NEFs to facilitate substrate release via an unknown mechanism. NEF: nucleotide exchange factor. B Domain topology of Hsmortalin and HsGrpEL1. MTS: mitochondrial targeting sequence. IDL: interdomain linker. C Representative 2-D classes of the HsmortalinR126W-GrpEL1 complex. Colored arrows correspond to the densities observed in (D). D DeepEMhancer-sharpened map of the HsmortalinR126W-GrpEL1 complex colored by subunits and subdomains. The structure represents the exchange intermediate depicted in (A).
