Fig. 4: HsmortalinR126W in complex with GrpEL1 is substrate-bound to a mortalin truncation product.

A Model of the HsmortalinR126W substrate binding domain (SBD) bound to a substrate fit into the DeepEMhancer-sharpened cryoEM map. B Rigid-body docking of the mortalin SBD into the posterior substrate EM density (low-pass filtered to 5 Å). C Residue mapping of the substrate within the mortalin substrate binding site. The density of the substrate is shown as a gray contour. D Comparison of the modeled Hsmortalin substrate with the crystal structure of EcDnaK bound to the NRLLLTG peptide (PDB: 4EZW).