Table 1 Marker genes from biofilm UMAP clusters, p-values are FDR-adjusted

0

sasA

SAUSA300_RS14395

Serine-rich repeat glycoprotein adhesin SasA

Virulence

Virulence

0.25

4.0E-15

pnpA

SAUSA300_RS06315

Polyribonucleotide nucleotidyltransferase

Transcription

Degradation of RNA

0.24

4.0E-15

-

SAUSA300_RS04570

Sodium:proton antiporter, TRAP transporter- DctM subunit

Transport and binding proteins

Carbohydrates, organic alcohols, and acids

0.20

4.4E-12

-

SAUSA300_RS09575

ATP-binding protein, DnaA regulatory inactivator Hda

DNA metabolism

DNA replication, recombination, and repair

0.16

5.6E-06

-

SAUSA300_RS06415

Energy coupling factor transporter S component ThiW

Biosynthesis of cofactors, prosthetic groups, and carriers; Transport and binding proteins

Thiamine; Other

0.16

2.3E-08

1

citB

SAUSA300_RS06765

Aconitate hydratase

Energy metabolism

TCA cycle

1.33

8.3E-66

ltaS

SAUSA300_RS03775

Lipoteichoic acid synthase

Cell envelope

Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides

1.28

3.2E-65

isdH

SAUSA300_RS09160

Iron-regulated surface determinant protein H

Transport and binding proteins

Cations and iron-carrying compounds

1.11

1.3E-48

-

SAUSA300_RS15340

RNase P RNA component class B

-

-

1.07

7.0E-43

ebpS

SAUSA300_RS07485

Elastin-binding protein EbpS

Virulence

Virulence

0.35

1.3E-05

2

rpoB

SAUSA300_RS02820

DNA-directed RNA polymerase subunit beta

Transcription

DNA-dependent RNA polymerase

6.43

<1.0E-323

-

SAUSA300_RS01265

Nucleoside hydrolase, translation initiation factor 2 gamma subunit

-

-

0.45

3.8E-09

-

SAUSA300_RS01440

MFS transporter

Cellular processes; Transport and binding proteins

Toxin production and resistance; Other

0.34

3.9E-02

rpsC

SAUSA300_RS12120

30S ribosomal protein S3

Protein synthesis

Ribosomal proteins: synthesis and modification

0.30

1.1E-02

ispA

SAUSA300_RS08025

Geranyltranstransferase

Biosynthesis of cofactors, prosthetic groups, and carriers

Menaquinone and ubiquinone

0.29

6.1E-04

3

clfB

SAUSA300_RS14270

Clumping factor B

Virulence

Virulence

6.75

<1.0E-323

cspB

SAUSA300_RS14655

Cold-shock protein

DNA metabolism; Cellular processes

DNA replication, recombination, and repair; Adaptations to atypical conditions

0.75

2.0E-06

arcA

SAUSA300_RS14295

Arginine deiminase

Energy metabolism

Amino acids and amines

0.50

9.6E-11

fnbB

SAUSA300_RS13525

Fibronectin-binding protein B

Virulence

Virulence

0.47

2.4E-05

-

SAUSA300_RS14010

Fructosamine kinase family protein

-

-

0.45

9.0E-08

4

qoxA

SAUSA300_RS05175

Quinol oxidase subunit 2

Energy metabolism

Electron transport

7.32

<1.0E-323

hemY

SAUSA300_RS09750

Protoporphyrinogen oxidase

Biosynthesis of cofactors, prosthetic groups, and carriers

Heme, porphyrin, and cobalamin

0.54

9.4E-03

ulaA

SAUSA300_RS01755

PTS ascorbate transporter subunit IIC

-

-

0.50

1.5E-02

bglR

SAUSA300_RS01375

GntR family transcriptional regulator

Regulatory functions

DNA interactions

0.38

7.3E-03

rplY

SAUSA300_RS02575

50S ribosomal protein L25/general stress protein Ctc

Protein synthesis

Ribosomal proteins: synthesis and modification

0.36

7.3E-03

5

gpmA

SAUSA300_RS13045

2,3-bisphosphoglycerate-dependent phosphoglycerate mutase

-

-

6.29

<1.0E-323

thiW

SAUSA300_RS05255

ABC transporter ATP-binding protein

Transport and binding proteins

Unknown substrate

2.28

6.5E-21

-

SAUSA300_RS12980

Zinc ABC transporter substrate-binding protein

Transport and binding proteins

Unknown substrate

1.72

1.1E-24

polX

SAUSA300_RS05610

DNA polymerase/3’−5’ exonuclease PolX

DNA metabolism

DNA replication, recombination, and repair

1.47

2.9E-26

fdaB

SAUSA300_RS14100

Class I fructose-bisphosphate aldolase

Biosynthesis of cofactors, prosthetic groups, and carriers

Other

1.46

2.4E-27

6

nrdE

SAUSA300_RS03845

Ribonucleotide-diphosphate reductase subunit alpha

Purines, pyrimidines, nucleosides, and nucleotides

2’-Deoxyribonucleotide metabolism

6.97

6.8E-233