Fig. 2: hACE2 transcript levels correlate with susceptibility to SARS-CoV-2.
From: Human ACE2 transgenic pigs are susceptible to SARS-CoV-2 and develop COVID-19-like disease
a Primary cells were generated from ear notches taken from transgenic pigs. Total RNA was harvested and hACE2 transcript levels determined by RT-qPCR. hACE2 levels were normalized to GAPDH and shown as relative expression. b To test susceptibility, fibroblast cells were infected at an MOI of 3. Supernatant was harvested 24 HPI and infectious virus quantified by TCID50. Data represents the mean from two independent experiments (each using two technical replicates). ND = not detected. c Correlation of SARS-CoV-2 titre and hACE2 expression levels are shown. Source data are provided as a Source Data file.
