Fig. 1: Cryo-EM structure of the dNTP-free RT-T/P complex.
From: Structural basis of deoxynucleotide addition by HIV-1 RT during reverse transcription
a Cryo-EM density map for the RT-T/P complex colored by structural subdomains. b Cartoon representation of the DNA polymerase active site of RT illustrating key protein motifs. The density for the T/P aptamer substrate is indicated in blue mesh with the corresponding model in the sticks. c Sequence of the 38-mer hairpin DNA aptamer T/P substrate used throughout this study. Nucleotides in positions +2 and +4 of the template strand labeled as Cm are 2′-O-methyl modified (2′-OMe). Inset, detail of the density for the 2′-O-methyl modified nucleotide in position +4. Map sharpened at B-factor −80 Å2 and contoured at 6σ.
