Fig. 6: NOS1APc recruits KANK1 to cell-cell junctions.
From: KANK1 promotes breast cancer development by compromising Scribble-mediated Hippo activation
a Tissue sections of KANK1-WT and KANK1-KO mammary glands (MG) and KANK1-WTPyMT and KANK1-KOPyMT tumors stained for SMA (green), NOS1APc-specific antibody (magenta) and CK8 (Cyan). Nuclei were counterstained with DAPI (blue). N = 4 independent repeats. Scale bars: 20 µm. b MCF7NOS1AP-KO cells were virally reconstituted with mCherry-NOS1APc (b1-2, b5-7) or mCherry only (b3-4) together with DOX-inducible KANK1-GFP expression construct (b1-4) or with DOX-inducible KANK1-∆UC-GFP (b5-6) and stained for Paxillin to highlight FAs at the bottom layer. The two cells not transduced with the mCherry-NOS1APc expression construct (asterisks, b1) displayed KANK1-GFP in the cytoplasm but not cell-cell contact. Black arrow heads in the line profile analysis (right panel) indicate the co-localization of KANK1-GFP and mCherry-NOS1APc at cell-cell junctions (n = 5 independent repeats). Scale bar: 20 µm. Source Data are provided as a Source Data file.
