Fig. 9: Junctional translocation of KANK1 promotes nuclear TAZ accumulation in malignant tissues.
From: KANK1 promotes breast cancer development by compromising Scribble-mediated Hippo activation
a, b Tumor sections from KANK1-WTPyMT and KANK1-KOPyMT mice stained for KANK1 or TAZ (magenta) and Nidogen (green). Arrow heads indicate BM-attached KANK1-WTPyMT tumor cells with predominantly cytoplasmic TAZ. Nuclei were counterstained with DAPI. Scale bars: 50 µm (a). The ratio of TAZ-positive nuclei per animal is shown in (b) (n = 5 for each group). P value was calculated using unpaired Student’s t-test (two-tailed). Mean ± SD is shown. c Tumoroids cultured in Matrigel were whole-mount stained for KANK1 (magenta) or NOS1APc-specific antibody (magenta) and Nidogen (green). Nuclei were counterstained with DAPI (blue). Repeats: n = 7 tumoroids for KANK1 and n = 8 for NOS1APc. Scale bars: 50 µm. d, e Tumoroids were whole-mount stained for TAZ (magenta) and CK8 (green). Maximal projection of the signals is shown. Nuclei were counterstained with DAPI. Scale bar: 50 µm (d). The ratio of TAZ-positive nuclei per organoid is shown in (e). Repeats: n = 13 for KANK1-WTPyMT and n = 11 for KANK1-KOPyMT. P value was calculated using unpaired Student’s t-test (two-tailed). Mean ± SD is shown. Source Data are provided as a Source Data file.
