Fig. 2: VL/VM responses can be classified into distinct M1-evoked patterns.
From: Preconfigured cortico-thalamic neural dynamics constrain movement-associated thalamic activity
a Average firing rates (z-scored) evoked by M1 stimulation for 300 ms (top) and 500 ms (bottom) ISI protocols (green and red dots). Cells were time sorted according to their highest firing rate between the first and second stimulus of the train. b Averaged population activity for the 300 ms, 500 ms, and surrogate data aligned to each stimulus of the train. Solid lines and shaded areas represent the median and the 25th and 75th percentiles, respectively. c Correlation distribution between each stimulus of the 300 ms and 500 ms trains and surrogate spiking activity (n = 377 cells x 5 stimuli; two-sided Mann–Whitney, *p < 0.001). Euclidean distances (d) and diagonal asymmetry index (e) between the relative and absolute 300/500 ms comparisons. Significant differences (two-sided Mann–Whitney, *p < 0.001) were calculated on observed and surrogated values from the 377 neurons that maintained stable conditions in 300 ms and 500 ms conditions. f Average M1-evoked patterns for cells classified as specific pattern clusters for the 300 ms and 500 ms conditions. Top and bottom rows display the full train and first stimulus magnification evoked patterns, respectively. Solid lines and shaded areas represent the median and the 25th and 75th percentiles, respectively. g Representative spike rasters and average peri-event histograms for neurons (same neuron in each column) belonging to specific pattern clusters recorded in the 300 ms (top row) and 500 ms (bottom row) conditions. Activity was aligned to the first stimulus of the train (green and red dots for 300 and 500 ms ISI, respectively). Maximum firing rates are indicated for each neuron. h Percentage of cells belonging to each pattern cluster displayed in (f) (upper panel, n = 377 neurons). Number of animals that presented each pattern (lower panel). K-W non-parametric one-way ANOVA, df = 9; X2 = 65.66; p < 0.001. The central line and box in boxplots (c–e, h) represent the median and 25th–75th percentiles, and whiskers extend to the most extreme datapoints excluding outliers.
