Fig. 1: Multiscale morphometry analysis from mouse brain images.
From: Neuronal diversity and stereotypy at multiple scales through whole brain morphometry
A The mouse brain dataset IMG204 comprises 204 brains (3.7 peta-voxels) of 3 different modalities (fMOST, STPT, and LSFM) obtained from 4 BICCN projects. Left, a multiplexing view displays salient voxels on the sagittal middle sections of six mouse brains from different sources. The salient voxels are colored by image sources. Middle, the CCFv3 atlas that all brains are registered to. Right, representative sagittal maximum intensity projections of whole-brain images from each modality and source. Imaging modality, research group, the number of brains collected, and typical voxel size are specified at the top. B Left, sagittal view of the spatial distribution of 182,497 semi-automatically annotated somas on the CCFv3 template, along with their densities (color bar). Each soma is represented by an individual dot. Right, horizontal projection of five regions (color-coded) along the anterior-posterior (AP) axis (left) and respective soma locations as dots (right). C Left, horizontal projection of auto-traced dendritic morphologies (SEU-D15K). Middle, dendritic microenvironment (M) representation for each neuron (target). A microenvironment is a spatially tuned average (see Methods) of the most topologically similar neurons (up to six neurons, including the target neuron) within a distance of 249 ÎĽm from the target neuron. Right, morphology of the target neuron within the microenvironment on the left. D Multiscale morphometry. Hierarchical representation including representative visualizations for six scales of morphometries ranging from centimeters to micrometers, i.e., neuron population (mouse lines and projection types), full morphology, arbor, motif, varicosity, and the microenvironment displayed in (C). E Heatmap of the cross-scale feature map for lamination subtypes of cortical neurons (s-type-layer). Soma types (s-types) with their soma located in the same cortical lamination were grouped together. Source data are provided as a Source Data file.
