Fig. 4: ASCL1 and OLIG2 inversely regulate glioma tumor cell types. | Nature Communications

Fig. 4: ASCL1 and OLIG2 inversely regulate glioma tumor cell types.

From: Transcription factors ASCL1 and OLIG2 drive glioblastoma initiation and co-regulate tumor cell types and migration

Fig. 4: ASCL1 and OLIG2 inversely regulate glioma tumor cell types.

Representative immunofluorescent images and H&E staining of terminal control (a–d), Ascl1-CKO (e–h), Olig2-CKO (i–l), dCKO (m–p), and Ascl1-OE (q–t) tumors. High magnification showing differences in co-localization, or lack thereof, of GFAP (white arrow) or SOX10 (white arrowhead) in reporter+ tumor cells. Dotted line delineates DAPI+ cell nuclei that overlap with tumor reporter and/or cell type markers. Similar staining was seen in n = 3/genotype. Black arrows and arrowheads in H&E panels indicate the presence of GFAP+ pink fibers and “fried-egg” cell shapes characteristics of astrocytoma and oligodendroglioma, respectively. Scale bars: 12.5 μm for (c, g, k, o, s) and 50 μm for all other panels.

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