Fig. 3: Heterologous HIV-1 neutralization profile of plasma antibodies elicited by neonatal and adult SHIV infection.
A–G Longitudinal plasma antibodies spanning 24 months following neonatal and adult SHIV infection were tested for neutralization of heterologous HIV-1 reference strains from the global panel. Neutralization titer was measured in TZM-bl cells and reported as ID50 (reciprocal dilution). The heatmaps show the neutralization titers of plasma antibodies in seven young RMs with induction of heterologous HIV-1 NAbs following neonatal SHIV infection (A–G), and their corresponding dams including two adult RMs with induction of heterologous HIV-1 NAbs (A–B). See methods for criteria of heterologous HIV-1 NAb induction as described29. Shown are titers against viruses neutralized by the plasma samples that met our criteria for the induction of heterologous HIV-1 NAb induction. As shown by the key at the top of the figure, the heatmap was color coded to show the magnitude of NAb titers generated in each animal at different timepoints against each virus tested. Adults V060 and BD62 died before month 24 post-SHIV infection. Asterisk (*); neutralization titer was less than 3X the negative control virus (MuLV).
