Fig. 1: An MP1 neuron to cortex glia Dh44 signalling axis inhibits glial fatty acid synthesis.
a BODIPY staining in the MB cortex region and quantification of LD mean area and density comparing genotypic control flies to flies expressing in adult cortex glia the Dh44-R1 RNAi (n = 18; mean area: t35 = 3.44 p = 0.0015; density: t35 = 3.44 p = 0.0015) or the Dh44-R2 RNAi (n = 9; mean area: t16 = 0.43, p = 0.67; density: n = 9, t16 = 0.52, p = 0.60). b BODIPY staining in the MB cortex region and quantification comparing flies expressing the Dh44-R1 RNAi alone, or Dh44-R1 RNAi and ACC RNAi together in adult cortex glia with genotypic control (n = 14; mean area: F2,39 = 12.88, p = 5 × 10−5; density: F2,38 = 19.20, p = 2 × 10−6). c The specific driver line MB320C allowed mCD8::GFP expression in MP1 neurons. Immunostaining against GFP (green) and Dh44 (magenta) revealed expression of Dh44 in MP1 neurons (merge), strongly reduced by co-expression of Dh44 RNAi (n = 9, t17 = 3.96, p = 0.001). d BODIPY staining in the MB cortex region and quantification comparing flies expressing the Dh44 RNAi in adult MP1 neurons with genotypic control (n = 13; mean area: t25 = 3.08, p = 0.005; density: n = 13, t21 = 2.16, p = 0.04). e BODIPY staining in the MB cortex region and quantification comparing flies expressing the Dh44 RNAi alone or both Dh44 RNAi and ACC RNAi, in adult MP1 neurons and cortex glia, with genotypic control (n = 14; mean area: F2,35 = 11.66, p = 0.0001; density: F2,35 = 8.12, p = 0.0013). RNAi lines KK108591 (Dh44-R1), JF03289 (Dh44-R2), GD3482 (ACC) and JF01822 (Dh44) were used in this figure. Data are represented as mean ± SEM. Scale bars indicate 5 µm (c) or 20 µm (a, b, d, e). ns: not significant, p > 0.05, *p < 0.05, **p < 0.01, ****p < 0.0001 by two-tailed Student’s t test (a, c, d) or Tukey’s pairwise comparison following one-way ANOVA (b, e). Source data are provided as a Source Data file.
