Fig. 6: The MP1 neuron to cortex glia Dh44 signalling axis is necessary for long-term memory.
a Dh44 KD in MP1 neurons impaired LTM measured after spaced training (n = 18, F2,51 = 9.22, p = 0.0004) but did not affect memory resulting from massed training (n = 12, F2,33 = 0.59, p = 0.55). Flies showed normal LTM after spaced training when RNAi expression was not induced (n = 11, F2,30 = 0.37, p = 0.68). b Dh44-R1 KD in cortex glia impaired LTM measured after spaced training (n = 12, F2,33 = 1.03, p = 1 × 10−7) but did not affect memory resulting from massed training (n = 12, F2,33 = 0.66, p = 0.67). Flies showed normal LTM after spaced training when RNAi expression was not induced (n = 10, F2,27 = 1.59, p = 0.22). c The dual Dh44-R1 and ACC KD in cortex glia rescued the LTM deficit after spaced training observed upon the single KD of Dh44-R1 (n = 12, F4,85 = 6.94, p = 0.000016). d Dh44 KD in both MP1 neurons and cortex glia caused an LTM defect after spaced training, which was rescued in the dual Dh44 and ACC KD condition (n = 18, F4,85 = 6.35, p = 0.0002). RNAi lines KK108591 (Dh44-R1), GD3482 (ACC) and JF01822 (Dh44) were used in this figure. Data are represented as mean ± SEM. ns: not significant, p > 0.05, ***p < 0.001 by Tukey’s pairwise comparison following one-way ANOVA. Source data are provided as a Source Data file.
