Fig. 4: MinDE-induced bleb morphologies on phase-separated GUVs with encapsulated actomyosin architectures.
From: Self-organized spatial targeting of contractile actomyosin rings for synthetic cell division
a Schematic illustration (top) and 3D confocal image (bottom) show the membrane composition employed to generate phase-separated vesicles and the domains obtained. Scale bar: 10 µm. b 3D projections and 2D confocal images depict a blebbing phase-separated vesicle. MinDE proteins bind and oscillate on Ld domains. Actomyosin bundles remain at lipid-phase boundaries as Min proteins transiently deform Ld domains (orange arrows). Inner encapsulation mix: 2.4 µM actin, 0.6 µM fascin (fascin/actin molar ratio = 0.25), 0.05 µM myosin II, 20 g/L Ficoll70, 3 µM MinD, 3 µM MinE and 5 mM ATP. Scale bars: 20 µm. c Schematic illustration of the proposed mechanism behind the dynamic deformation of Ld domains by MinDE protein oscillations.
